NAD⁺ biology and the twelve domains that define your biological baseline.
Begin the AssessmentFive minutes. The Assessment is free. Pricing appears only if your biology qualifies.
What our Foundation Input supplement is. What NAD⁺ does. Why measurement comes first.
Our Foundation Input supplement is a direct precursor to NAD⁺ — a coenzyme essential to mitochondrial energy production, sirtuin-mediated cellular repair, PARP-mediated DNA repair, and circadian regulation.
NAD⁺ levels decline measurably with age. That decline is associated with patterns many people recognize — energy that drops earlier in the afternoon, sleep that feels less restorative, mental clarity that fluctuates more than it used to, skin that loses resilience and recovers more slowly from daily stress, physical recovery that takes longer after exertion, metabolic consistency that becomes harder to maintain. These are not isolated complaints. They map to biological systems where NAD⁺ availability has a documented role — and they are exactly what the twelve domains of the Cellular Vitality Index™ are designed to observe and document.
That is why measurement comes first. Without a documented baseline, a 100-day observation has no reference point. The Assessment establishes where you are. The protocol documents what changes.
Twelve biological domains. Each one connected to published NAD⁺ research — and each one connected to something most adults can recognize in their own daily experience.
The Cellular Vitality Index™ does not score your skin, your age, or your habits. It documents self-reported biological signal across twelve domains where NAD⁺ availability has a documented role in published research. Each domain corresponds to patterns many people notice in themselves — afternoon energy, sleep quality, mental clarity, recovery time — translated into a structured biological frame.
Dermal Resilience
NAD⁺ supports sirtuin and PARP enzyme activity involved in skin cell repair and extracellular matrix maintenance.
WEIGHT
12%
Cellular Energy Expression
NAD⁺ is essential to the mitochondrial electron transport chain — declining availability reduces cellular ATP production efficiency.
WEIGHT
12%
Sleep Architecture
NAD⁺ regulates circadian rhythm through SIRT1-mediated control of the CLOCK/BMAL1 transcription complex.
WEIGHT
12%
Physical Recovery
NAD⁺ supports mitochondrial biogenesis and PARP-mediated DNA repair in muscle cells following physical exertion.
WEIGHT
12%
Metabolic Equilibrium
NAD⁺ is a required cofactor for enzymes in glycolysis and the citric acid cycle — influencing energy consistency across the day.
WEIGHT
8%
Cognitive Steadiness
NAD⁺ availability in neural tissue supports mitochondrial energy production and neuroprotective sirtuin activity.
WEIGHT
8%
Skin Luminosity & Tone
NAD⁺ supports mitochondrial energy in keratinocytes and regulates sirtuin-mediated cellular turnover in surface skin layers.
WEIGHT
8%
Cardiovascular Ease
NAD⁺ supports endothelial function through eNOS activity and SIRT1-mediated vascular biology.
WEIGHT
8%
Vitality Architecture
NAD⁺ supports SIRT1-mediated regulation of steroid hormone biosynthesis — pathways connected to physical drive and hormonal equilibrium.
WEIGHT
8%
Ocular Surface Comfort
Published research documents NAD⁺’s role in meibomian gland cell function, which governs tear film stability.
WEIGHT
4%
Immune Resilience
NAD⁺ aids DNA repair in immune cells and provides energy for rapid immune cell proliferation and response.
WEIGHT
4%
Stress Adaptation
NAD⁺ supports circadian stability and hypothalamic NAD⁺ biosynthesis — pathways connected to stress response regulation.
WEIGHT
4%
Each pillar is connected to documented NAD⁺ biology.
The research foundation.
The research supporting the twelve domains and the Foundation Input supplement draws from three tiers of evidence — each with a different relationship to what participants may observe in their own biology.
Direct Human Evidence
Multiple randomized controlled trials in adults have documented NAD⁺ bioavailability following oral direct NAD⁺ precursor supplementation, alongside measurements of insulin sensitivity, walking endurance, and metabolic markers. Trials have been conducted across age ranges from 40 to 80 and across dosage levels comparable to the protocol used in RL100. This is the evidence tier most directly relevant to what participants in RL100 may document across their window.
Observational Human Evidence
Age-related NAD⁺ decline has been documented across multiple human cohort studies. Tissue-level NAD⁺ measurements correlate with biological age markers, mitochondrial function indicators, and physiological reserve across organ systems. This research establishes the biological context — why NAD⁺ decline is a measurable phenomenon and why the twelve domains were selected.
Preclinical Evidence
Mechanistic and animal model studies establish the biological pathways through which NAD⁺ operates across the twelve measured domains — SIRT1-mediated regulation, PARP enzyme activity, mitochondrial biogenesis, and circadian gene expression. This layer explains the mechanisms — why the biology works the way the research suggests it does.
Begin with measurement.
The Assessment is free. It takes five minutes. It establishes your biological baseline across twelve domains — and tells you whether the structured supplement protocol is appropriate for your biology.
Begin the AssessmentFree. Five minutes. Pricing appears only if your biology qualifies.